The development of flexible transparent conductive films for optoelectronic applications requires materials capable of simultaneously providing adequate electrical conductivity, optical transmittance, and mechanical stability. In this study, expanded graphite (EG) was employed to fabricate a flexible transparent conductive film on a polyethylene terephthalate (PET) substrate using a rubbing mechanical transfer method. The pristine graphite was subjected to acid intercalation followed by thermal expansion at 950 °C and subsequently transferred onto PET. The transfer process was performed under a normal force of 25 N, a frequency of 2.5 Hz, and a stroke length of 100 mm. The structural and morphological changes in the samples at different stages of the process were investigated using X-ray diffraction (XRD), Raman spectroscopy, Fourier-transform infrared spectroscopy (FTIR), and field-emission scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (FE-SEM/EDX). Thermal expansion increased the interlayer spacing of graphite from 0.335 to 0.365 nm and increased the ID/IG ratio from 0.12 to 0.95. The resulting EG/PET film exhibited a sheet resistance of 500 Ω/sq and an optical transmittance of 70% at a wavelength of 550 nm, whereas pristine graphite exhibited a sheet resistance higher than 50 MΩ/sq and an optical transmittance of 35%. The Haacke figure of merit for the EG/PET film was calculated to be 5.65×10-5 Ω⁻¹. In the cyclic bending test, the film exhibited a relative resistance change of less than 4.5% after 1000 cycles at a bending radius of 10 mm. Comparison of pristine graphite and EG demonstrated that thermal expansion prior to mechanical transfer provides more favorable conditions for the formation of a conductive and relatively transparent carbon network on PET. The obtained performance, together with the electrical stability of the film under bending, indicates the potential of this approach for the fabrication of flexible transparent conductive films.