Journal of Textile Science and Technology

Journal of Textile Science and Technology

Development of Antibacterial Cotton Wound Dressings Using Kamala Natural Dye and Bio-Mordants: Evaluating the Efficacy of Mordanting Techniques Against Nosocomial Pathogens

Document Type : Research/ Original/ Regular Article

Authors
Fatemeh Saeedfar 1
Fatemeh Shahmoradi Ghaheh 2
Sayyedeh Rahmaneh Atyabi 3
Zahra Sadeghi 3
1 Imam Mohammad Bagher (AS) Senior High School
2 Department of Environment, Urmia University of Technology, Urmia, Iran
3 Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Hezar-Jereeb Street, 81746-73441, Isfahan, Iran
10.22034/jtst.2026.588139.1557
Abstract
Infections caused by bacteria such as Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli are among the factors threatening the health of patients. Conventional cotton wound dressings lack antimicrobial properties and provide suitable conditions for bacterial growth, leading to delayed wound healing, increased risk of secondary infections, and antibiotic resistance. The use of wound dressings containing plant pigments with antibacterial properties has been considered as a novel approach for infection control. The aim of this study was to use a natural dye extracted from the fuzz of Kamala fruit (agricultural waste) for dyeing cotton bandages and gauze. This plant has high antimicrobial activity due to its phenolic and tannin compounds. In order to enhance dyeability and increase antibacterial activity, aqueous extracts of black tea, plantain, pomegranate peel, black myrobalan, and cumin were used as bio-mordants in both pre-mordanting and post-mordanting methods. The results showed that samples dyed with pomegranate peel, plantain, and black tea extracts had effective antibacterial activity against S. aureus and P. aeruginosa. The highest inhibitory effect was observed in samples that were first dyed with Kamala natural dye and then mordanted with plantain and pomegranate peel. These findings demonstrate the high potential of the studied plant compounds for producing antibacterial wound dressings and their therapeutic applications.
Keywords
Kamala
Antibacterial properties
Cotton wound dressing
Green synthesis
Hospital infections
Subjects
1. Fattahi, F. and Zamani, T. (2019). Investigation into the Effects of Nano-fibrous Wound Dressings on Wound Healing Process in Animal Models, Journal of Textile Science and Technology, 8(4), 29-48. doi: 20.1001.1.21517162.1398.8.4.3.0
2. Khan, R., Xiangyang, S., Ahmad, A., and Mo, X., (2018). Electrospinning of crude plant extracts for antibacterial and wound healing applications: a review, SM Journal of Biomedical Engineering, 4(1), 1024.
3. Desai, J., Chauhan, J., Mankad, A., and Maitreya, B., (2023). Natural colourants: A review, International Association of Biologicals and Computational Digest, 2(1), 261-270. https://doi.org/10.56588/iabcd.v2i1.121
4. Naveed, R., Bhatti, I. A., Adeel, S., Ashar, A., Sohail, I., Khan, M. U., Masood, N., Iqbal, M., and Nazir, A., (2022). Microwave assisted extraction and dyeing of cotton fabric with mixed natural dye from pomegranate rind (Punica granatum L.) and turmeric rhizome (Curcuma longa L.), Journal of Natural Fibers, 19(1), 248-255. https://doi.org/10.1080/15440478.2020.1738309
5. Shojaeizad, S., Tavakoli, R. A., and Sheikholeslami, M. A., (2020). Investigation of the properties of chitosan film containing nettle extract, Research Journal of Shahid Beheshti University of Medical Sciences, 44(3), 454-459. http://pejouhesh.sbmu.ac.ir/article-1-2028-fa.html
6. Kumar, R., Tripathi, Y. C., and Kaushik, P. K., (2016). Process optimization for extraction of natural dye from M. philippinensis fruits and its application on different fabrics, World Journal of Pharmaceutical Research, 5(4), 927-945. https://doi.org/10.20959/WJPR20164-5876
7. Kumar, A., Patil, M., Kumar, P., Bhatti, R. C., Kaur, R., Sharma, N. K., and Singh, A. N., (2021). Mallotus philippensis (Lam.) Müll. Arg.: A review on its pharmacology and phytochemistry, Journal of Herbmed Pharmacology, 10(1), 31-50. https://doi.org/10.34172/jhp.2021.03
8. Hewageegana HGSP and Hewageegana AU, Medicinal Potential and Scientific Validations of Mallotus philippinensis (Lam.) Muell. Arg. (Kampillaka): A Review, Journal of Natural & Ayurvedic Medicine, 5(3), 1-17. https://doi.org/10.23880/jonam-16000314
9. Gangwar, M., Goel, R. K., and Nath, G., (2014). Mallotus philippinensis Muell. Arg (Euphorbiaceae): ethnopharmacology and phytochemistry review, BioMed Research International, 2014(1), 213973. https://doi.org/10.1155/2014/213973
 
10. Tehrani, M., Shahmoradi Ghaheh, F., and Jahanbazi, S., (2023). Dyeing silk fabric using anthocyanin pigments extracted in different solvents from Hibiscus sabdariffa L, Journal of Textile Science and Technology, 12(1), 27-39. dor: 20.1001.1.21517162.1402.12.1.3.2
11. F. Shahmoradi Ghaheh, M.K. Moghaddam, M. Tehrani. (2021). Comparison of the effect of metal mordants and bio‐mordants on the colorimetric and antibacterial properties of natural dyes on cotton fabric. Coloration Technology,6:137,689-698
https://doi.org/10.1111/cote.12569
12. A. Haji, F. Shahmoradi Ghaheh, L. Mohammadi. (2023). Dyeing of polyamide 6 fabric with new bio-colorant and bio-mordants. Environmental Science and Pollution Research,13:30,37981-37996. https://doi.org/10.1007/s11356-022-24862-0
13. Clinical and Laboratory Standards Institute (CLSI),  (2024). Performance Standards for Antimicrobial Disk Susceptibility Tests. 14th ed. CLSI document M02-Ed14. Wayne, PA
14. Kumar, A., Patil, M., Kumar, P., Bhatti, R. C., Kaur, R., Sharma, N. K., and Singh, A. N., (2020). Mallotus philippensis (Lam.) Müll. Arg.: A comprehensive review on ethnomedicinal uses, Ethnobiology and Conservation, 9, 1-15. https://doi.org/10.15451/ec2020-05-9.19-1-44
15. Kamali Moghaddam, M., (2022). Principles of Textile Dyeing, University of Bonab.
16. Alqahtani, A., Mohamed, A.A., Ahmad, I., Alraey, Y., Asiri, M., Kopel, J., and Alshahrani, M.M., (2023). Pomegranate peel extract is an effective agent against MDR bacteria, Cellular and Molecular Biology, 69(5), 6-11.  https://doi.org/10.14715/cmb/2023.69.5.2   
17. Saini, S., Mishra, P., Balhara, M., Dutta, D., Ghosh, S., and Chaudhuri, S., (2023). Antimicrobial potency of Punica granatum peel extract: Against multidrug resistant clinical isolates, Gene Reports, 30, 101744. https://doi.org/10.1016/j.genrep.2023.101744  
18. Ali, A., Chen, H., Xu, H., Wang, S., and Yao, S., (2024). Accessing the medicinal potential of Mallotus philippensis: Comprehensive exploration of antioxidant and antibacterial properties through phytochemical analysis and extraction techniques, Separations, 11(6), 165. https://doi.org/10.3390/separations11060165 19. Jafari, H., Ghaffari-Bohlouli, P., Niknezhad, S.V., Abedi, A., Izadifar, Z., Mohammadinejad, R., Varma, R.S., and Shavandi, A., (2022). Tannic acid: a versatile polyphenol for design of biomedical hydrogels, Journal of Materials Chemistry B, 10(31), 5873-5912. https://doi.org/10.1039/d2tb01056a   
20. Alegbe, E.O., and Uthman, T.O., (2024). A review of history, properties, classification, applications and challenges of natural and synthetic dyes, Heliyon, 10(13), e33678. https://doi.org/10.1016/j.heliyon.2024.e33646  
21. Bodas, K. S., Bagul, C. D., and Shinde, V. M., (2022). Evaluation of wound healing effect of Mallotus philippensis (Lam.) Mull. Arg. by in silico multitargets directed for multiligand approach, In Silico Pharmacology, 10(1), 19. https://doi.org/10.1007/s40203-022-00134-0
22. Akiyama, H., Fujii, K., Yamasaki, O., Oono, T., and Iwatsuki, K., (2001). Antibacterial action of several tannins against Staphylococcus aureus, Journal of Antimicrobial Chemotherapy, 48(4), 487-491. https://doi.org/10.1093/jac/48.4.487
23. Łojewska, E. and Sakowicz, T., (2021). An alternative to antibiotics: selected methods to combat zoonotic foodborne bacterial infections, Current Microbiology, 78(12), 4037-4049. https://doi.org/10.1007/s00284-021-02665-9
24. Biharee, A., Sharma, A., Kumar, A., and Jaitak, V., (2020). Antimicrobial flavonoids as a potential substitute for overcoming antimicrobial resistance, Fitoterapia, 146, 104720. https://doi.org/10.1016/j.fitote.2020.104720
25. Grzywaczyk, A., Rojewska, M., Smułek, W., McNaughton, D. A., Prochaska, K., Gale, P. A., and Kaczorek, E., (2025). Glycyrrhiza glabra L. saponins modulate the biophysical properties of bacterial model membranes and affect their interactions with tobramycin, Langmuir, 41(18), 11701-11710. https://doi.org/10.1021/acs.langmuir.5c00927
26. Jing, W., Guo, D., Ning, Z., Yang, Y., Liu, T., Wang, M., and Gao, H., (2022). New polyphenolic glycosides from the stems of Caesalpinia cucullata and their inhibitory effect on methicillin-resistant Staphylococcus aureus with different ways, Bioorganic Chemistry, 129, 106193. https://doi.org/10.1016/j.bioorg.2022.106193

  • Receive Date 22 June 2026
  • Accept Date 27 June 2026