The textile industry is‏ ‏one of the major environmental polluters. It has been increased the researcher interest to explore eco-‎friendly ways and replace hazardous‏ ‏chemical materials with natural products. In the present work, for the first time, Z. Spina-‎christ root and Sichka gall plant by-products were employed for sustainable dyeing of wool yarns. Sichka is an asexual gall that ‎results from the sting of the wasp Andricus curtisii‏‎ ‎(Cynips mediterranea Trott)‎‏ ‏‎ on the buds of the oak (Quercus infectoria). ‎Bio-compounds of Z. Spina-christ and gall were extracted by ultrasonic method and the extracts were used as a natural dye and ‎bio-mordant for the dyeing process, respectively. Response surface method and central composite design were used to evaluate ‎the effect of the main process parameters and dyeing optimization. Results showed that the selected materials contain phenolic ‎and tannin bio-constitute and have a sufficient ability to use in the eco-dyeing process of textiles. The analysis of the variance of ‎the quadratic model showed that the dye concentration, bio-mordant concentration, and pH were significant model terms and had ‎the highest effects on the dyeing performance, respectively. Results indicate that for the conditions used in our experiments, the ‎optimum dyeing conditions are dye conc. =96%, bio-mordant conc. =12%, and pH=7.5. Dye fiber bonding stability of the dyed ‎samples was‏ ‏evaluated in terms of colorfastness to wash and light. Using Sichka as a bio- mordant in the dyeing process with Z. ‎Spina-christ root extract can improve the color strength of wool yarns and obtain acceptable dye fastness properties. As a result, ‎Z. Spina-christ root and Sichka bio-wastes, may, thus, be considered good replacements for the toxic dyes and mordants, ‎especially because they improve the natural dyeing efficiency of wool yarns without adverse environmental effects and produce ‎biodegradable byproducts with value-added properties.‎