مطالعه خواص سلولز باکتریایی حاوی دندریمر ، مواد گیاهی و شیمیایی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه نساجی ،دانشکده فنی مهندسی ، دانشگاه آزاد اسلامی واحد یادگار امام خمینی (ره) شهرری، تهران ، ایران

2 گروه نساجی ، دانشکده فنی مهندسی ، دانشگاه آزاد اسلامی واحد یادگار امام خمینی (ره) شهرری

چکیده

ویژگی های منحصر به فرد نانو ساختار ظریف سلولز باکتریایی تولید شده توسط استوباکتر گزیلینوم سبب تحقیقات زیادی در جهت تولید یک پانسمان ایده آل از این ترکیب شده است. هدف اصلی این مطالعه ، بررسی اثر سلولز باکتریایی حاوی دندریمر و عصاره های گیاهی (حنا، چای سبز، پنیرک صحرایی) و مواد شیمیایی( پنی سیلین، لیدوکایین و اسید گلیکولیک) بر روی باکتری های استافیلوکوکوس ارئوس( گرم مثبت) و سودوموناس آئروژینوزا ( گرم منفی) می باشد. در این مطالعه دیسک های سلولزی تهیه شده از سلولز باکتریایی ،با 0.5 درصد دندریمر تکمیل شدند. سپس سلولز میکروبی خام در مدت زمان 48 ساعت در دمای اتاق با مواد گیاهی و شیمیایی غوطه ور شد. نتایج ضد میکروبی به صورت هاله عدم رشد پس از 24 ساعت در برابر باکتری های مورد نظر اندازه گیری شد. میزان رهایش مواد موثره از سلولزباکتریایی در آب و بافر فسفات، آنالیز میکروسکوپی SEM و طیف سنجیFTIR انجام شد. تمام نمونه های تکمیل شده با دندریمر و مواد موثره گیاهی و شیمیایی دارای هاله عدم رشد در محیط استافیلوکوکوس ارئوس و نمونه تکمیل شده با اسید گلیکولیک دارای هاله عدم رشد در برابر سودوموناس آئروژینوزا بود. رهایش تمام مواد در بافر فسفات بیشتر از آب بود. نتایج میکروسکوپ SEM نمونه سلولز میکروبی خالص، دارای یک ساختار شبکه سه بعدی بسیار متخلخل را نشان داد. نتایج آنالیزFTIR گروههای عاملی روی سطح و پیوند بین دندریمر، سلولز میکروبی و دندریمر-مواد گیاهی- مواد شیمیایی را نشان داد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Study on the Properties of Bacterial Cellulose Containing Dendrimers, Plant and Chemical Materials

نویسندگان [English]

  • reyhaneh Azarmi 1
  • Ali Ashjaran 2
  • Shirin Nourbakhsh 2
  • Azam Talebian 2
1 Textile Department , Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch
2 Textile Department , Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University ,Tehran, Iran
چکیده [English]

The unique properties of the bacterial cellulose nanostructure produced by Acetobacter xylinus are the reason of extensive research to produce an ideal wound dressing. The aim of this study was to evaluate antibacterial effect of bacterial cellulose finished with dendrimer and herbal extract ( henna, green tea, malva sylvestris) and chemical pharmaceutical compounds )penicillin, lidocaine, glycolic acid) on Staphylococcus aureus) Gram positive( and Pseudomonas aeruginosa ) Gram negative )bacteria. Cellulose disks prepared from bacterial cellulose were finished with 0.5% dendrimer. The bacterial cellulose was then immersed in herbal extracts and chemical materials for 48 hours at room temperature. Antimicrobial results were measured as a disk diffusion after 24 hours. Drug delivery of herbal extract and chemical pharmaceutical compounds from bacterial cellulose in water and phosphate buffer, microscopic analysis)SEM) and spectroscopy FTIR were evaluated. All samples treatment with dendrimers and herbal and chemical compound had a growth inhibition zone on Staphylococcus aureus bacteria and the bacterial cellulose treatment with glycolic acid had a growth inhibition zone against Pseudomonas aeruginosa. Drug delivery of all materials in phosphate buffer was more than water. Scanning Electron Microscopy (SEM) results showed that pure bacterial cellulose samples had a highly porous three-dimensional network. The results of the FTIR analysis indicated functional groups on the surface, chemical bond between bacterial cellulose and dendrimer, dendrimer and herbal extract, and chemical pharmaceutical.

کلیدواژه‌ها [English]

  • Staphylococcus aureus -Pseudomonas aeruginosa
  • -bacterial cellulose
  • -disc diffusion
  • -herbal extract
  • - chemical pharmaceutical
1) Li Y, Jiang H, zheng W, Gong N, Chen L, Jiang X and Yang G, Bacterial cellulose-hyaluronan nanocomposite biomaterials as wound dressing for severe skin injury repair, 3(17), 3498-3507,2015
2) Lin W, Lien Ch, Yeh H, Yu Ch , Hsu Sh, Bacterial cellulose and bacterial cellulose–chitosan membranes for wound dressing applications, Carbohydr Polym, 94(1), 603-605, 2013.
3) Pang M, Huang Y,Meng F,Zhuang Y,Liu H,Manling Du,Qianqian Ma,Wang Q ,Chen Zh ,ChenL ,CaiT,Cai Y, Application of bacterial cellulose in skin and bone tissue engineering,  European Polymer Journal, 122( 5), 2020,
4) Lin Q , Jiang G & Tong K , Designed Monomers and Polymers, Dendrimers in Drug-Delivery Applications Designed Monomers & Polymers, 13(4):301-324,2012.
5) Tripathy S and Malay K , Dendrimers and their Applications as Novel Drug Delivery Carriers, Journal of Applied Pharmaceutical Science, 3 (09), 142-149, 2013.
6) Winnicka K , Wroblewska M , Wieczorek P, Tomasz Sacha P and Tryniszewska E , The Effect of PAMAM Dendrimers on the Antibacterial Activity of Antibiotics with Different Water Solubility, Molecules, 18(2), 8607-8617,2013.
7) Khaled S , Fuad A , Khaldoon O, Alshakka M, Phytochemical Screening And Antibacterial Activity Of Yemeni.Henna (Lawsonia Inermis) Against Some Bacterial Pathogens, Journal of Pharmacy and Biological Sciences, 11(2), 24-27,2016.
8) Ohishi T , Gotob Sh, Monirac p , Isemura b,c M, and Nakamura Y ,Anti-inflammatory Action of Green Tea, Antiinflamm Antiallergy Agents Med Chem, 15(2),74-90,2016.
9) Sadat Sh, Ahani Azari A, Mazandarani M, Evaluation of the Antibacterial Activity of Ethanolic Extract of Matricaria chamomilla, Malva sylvestris, and Capsella bursa-pastoris against Methicillin Resistant Staphylococcus aureus, Journal of Medical Microbiology and Infectious Diseases, J Med Microbiol Infect Dis, 8 (4), 127-131,2020.
10) Hermanns H , W. Hollmann M, F. Stevens M , Lirk Ph , Brandenburger T, Piegeler T and Werdehausen R, Molecular mechanisms of action of systemic lidocaine in acute and chronic pain, ,2019, British Journal of Anaesthesia, 123(3):335-349,2019
11) Schmidt RM, Rosenkranz HS. Antimicrobial activity of topical anesthetics: Lidocaine and procaine. J lnfect Dis. 121:597-607,1970.
12) Hashim P, The effect of Centella asiatica, vitamins, glycolic acid and their mixtures preparations in stimulating collagen and fibronectin synthesis in cultured human skin fibroblast Pak J Pharm Sci, 27(2):233-237,2014.
13) Lobanovska M and Pilla G, Penicillin’s Discovery and Antibiotic Resistance: Lessons for the Future, Journal Of Biology And Medicine, 90 (3), 135-145,2017.
14) خاکزار بفرویی فاطمه ، , محمدعلی مالک رضا ، مظاهری فیروزمهر ، تاثیر دندریمر بر قابلیت رنگرزی پارچه پنبه ای با مواد رنگزای مستقیم، 6 (1) ، 59-65 ، 1391
15) Chis A, Dobrea C, Morgovan CL, Maria Arseniu A, Liviu Rus L , Butuca A, Maria Juncan A , Maria Totan, Andreea Loredana Vonica-Tincu, Gabriela Cormos, Andrei Catalin Muntean, Maria Lucia Muresan, Felicia Gabriela Gligor and Adina Frum, Applications and Limitations of Dendrimers, Molecules , 25 (7 ), 1- 41 ,2020.
16) Gorgieva S, Bacterial Cellulose as a Versatile Platform for Research and Development of Biomedical Materials, processes, 8 (5), 1-26 ,2020.
17) Faustino Jozala A & Aparecida R , Pertile N & Alves dos Santos C & de Carvalho Santos-Ebinuma V& Martins Seckler M & Miguel Gama F & Pessoa Jr A, Bacterial Cellulose Production by Gluconacetobacter Xylinus By Employing Alternative Culture Media, Appl Microbiol Biotechnol: 99(3) , 1181-1190, 2015.
18) Bafrooei KH , Malek M.R, Mazaheri F, The Effect Of Dendrimer on Cotton Dye ability With Direct Dye, Chem. Ind. Chem. Eng. Q. 20 (3) 379−385, 2014
19) Ashjaran A, Sheybani S, Drug Release Of Bacterial Cellulose As Antibacterial Nano Wound Dressing, International Journal of Pharmaceutical Research & Allied Sciences: 137-143, 8(3), 2019.
20) Matuschek E, Brown D.F..G and Kahlmeter G, Development of the Eucast Disk Diffusion Antimicrobial Susceptibility Testing Method and Its Implementation. In Routine Microbiology Laboratories, Clinical Microbiology and Infection: 20(4), 255-266, 2014.
21) Ghosh S, Yadav S, Vasanthan N, Sekosan G. A study of antimicrobial property of textile fabric treated with modified dendrimers, 717-720, 2009.
22) Gallien J, Bhairavi S,   Rossignol J. PAMAM dendrimer nanomolecules utilized as drug delivery systems for potential treatment of glioblastoma, Int J A Systematic Review, 15: 2789-2808 , 2020.
23) Sasagawa K, D Hisanori , Sakagami R , Hirayama S , Maekawa T , Isono T , Hiyoshi T , Tamura H , Takizawa F , Fukushima Y , Tabeta K and Terao Y, Green Tea Exhibits Bactericidal Activity against Streptococcus pneumoniae and Inhibits Functional, Antibiotics , 10, 1550, 2021.
24) Chu DD and Juneja LR, General chemical composition of green tea and its infusion, in Chemistry and Applications of Green Tea, ed by Yamamoto T, Juneja LR, Chu DC and Kim M. CRC Press, Boca Raton, FL, pp 13–22 , 1997.
25) Rahman M , Alam Tumpa A , Zehravi M , Sarker MD , Yamin MD , Rezaul Islam Md , Harun-Or-Rashid Md , Ahmed M , Ramproshad S , Mondal B , Dey A , Damiri F , Berrada M , Habibur Rahman Md, and Cavalu s, An Overview of Antimicrobial Stewardship Optimization: The Use of Antibiotics in Humans and Animals to Prevent Resistance Md. Antibiotics, 11, 667, 2022.
26) Fernandes S, Inês B. Gomes, Manuel Simões, Antimicrobial activity of glycolic acid and glyoxal against Bacillus cereus and Pseudomonas fluorescens, Journal pree-proofs, 2-5, 2020.
27)  Ehrenreich A ,  Hornero J , Inés M, Dueñas J,  Cañete Rodríguez A, Gluconic acid: Properties, production methods and applications—An excellent opportunity for agro-industrial by-products and waste bio-valorization, Procees Biochemisty, 51(12), 1891-1903, 2016.
 
28) Prabal K. Maiti, Tahir C¸ agˇın Shiang-Tai Lin And William A. Goddard, Effect of Solvent and PH on the Structure of PAMAM Dendrimers, Macromolecules , 38, 979-991,2005.
29) Jung M. R. et al., “Validation of ATR FT-IR to identify polymers of plastic marine debris, including those ingested by marine organisms,” Marine Pollution Bulletin, 704–716, 2018.
30)Nandiyanto A.B.D , Oktiani R, and Ragadhita R, “How to read and interpret ftir spectroscope of organic material,” Indonesian Journal of Science and Technology, 4 (1), 97–118, 2019.
31) Javidparvar A. A., Naderi R., and Ramezanzadeh B., “L-cysteine reduced/functionalized graphene oxide application as a smart/control release nanocarrier of sustainable cerium ions for epoxy coating anti-corrosion properties improvement,” Journal of Hazardous Materials, 389, 122135, 2020.
32) Wang Q Q, Chen D J. Synthesis and Characterization of a Chitosan Based Nanocomposite Injectable Hydrogel. Carbohydr. Polymer, 136: 1 228-1 237,2016.