نوع مقاله : مقاله پژوهشی
نویسندگان
1 پژوهشکده بیوتکنولوژی، سازمان پژوهش های علمی و صنعتی ایران
2 سازمان پژوهش های علمی و صنعتی ایران، پژوهشکده بیوتکنولوژی‎ ‎
3 پژوهشکده بیوتکنولوژی، سازمان پژوهش های علمی و صنعتی ایران،
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Cartilage damages, including degenerative joint diseases, are a current medical problem. Once damaged, adult human articular cartilage has a limited capacity for intrinsic repair. Tissue engineering is becoming promising for cartilage repair. Nano fibers have considered as suitable candidates for improving the tissue engineering scaffold, since they can mimic microarchitecture of tissue extracellular matrix, perfectly. In this research, a biodegradable nanofiber scaffold of 10% silk fibroin and 5% chitosan solution with a ratio of 70-30 or 50-50 respectively, made by electrospinning technique for articular cartilage tissue engineering. By Scanning electron microscope imaging, nanofibers morphology and average diameter were determined. Fourier Transform Infrared spectroscopy was performed to investigate the functional groups of the scaffold surface. Mechanical properties of these scaffolds were determined by measuring of Young´s Module, Elongation at break and Breaking tenacity. PBS buffer was used for in-vitro degradation assay. Scanning electron microscope images analysis showed that the fibers were bead-less with uniform morphology with an average diameter of 148.8 ± 52.32 and 138.5± 24.51 nm respectively for 70-30 and 50-50 scaffolds. After 12 weeks assay, the percentage of degradation for 70-30 and 50-50 scaffold respectively were 6.16 ± 0.9 and 6.82±0.7% of total scaffold weight. As a result, silk fibroin-chitosan nanofiber scaffolds due to containing cell adhesion domains caused by their protein nature and because of their slow degradation, suitable physical structure and mechanical properties are suitable candidates for articular cartilage repair.
کلیدواژه‌ها [English]
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