Synthesis of silk fibroin‏-‏kitosan nanofibers as tissue engineering scaffold ‎for articular cartilage

Document Type : Research/ Original/ Regular Article

Authors

1 Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran‎

2 Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST)‎

Abstract

Cartilage damages, including degenerative joint diseases, are a current medical‏ ‏problem. Once damaged, adult ‎human articular cartilage has a limited capacity‏ ‏for intrinsic repair. Tissue engineering is becoming promising for ‎cartilage‏ ‏repair. Nano fibers‏ ‏have considered as suitable candidates for improving the tissue engineering‏ ‏scaffold, ‎since they can mimic microarchitecture of tissue extracellular matrix, ‎perfectly. In this research, a biodegradable ‎nanofiber scaffold of 10% silk fibroin and 5% ‎chitosan solution with a ratio of 70-30 or 50-50 respectively, made by ‎electrospinning technique ‎for articular cartilage tissue engineering. By Scanning electron microscope imaging, ‎nanofibers morphology and average ‎diameter were determined. Fourier Transform Infrared spectroscopy was ‎performed to ‎investigate the functional groups of the scaffold surface. Mechanical properties of these ‎scaffolds were ‎determined by measuring of Young´s Module, Elongation at break and Breaking ‎tenacity. PBS buffer was used for ‎in-vitro degradation assay. Scanning electron microscope images analysis showed that ‎the fibers were bead-less with ‎uniform morphology with an average diameter of 148.8 ± 52.32‎‏ ‏and 138.5± 24.51 nm respectively for 70-30 and 50-‎‎50 scaffolds. After 12 weeks‎‏ ‏assay, the percentage of degradation for 70-30 and 50-50 scaffold respectively‎‏ ‏were ‎‎6.16 ± 0.9 and 6.82±0.7% of total scaffold weight. As a result, silk fibroin-chitosan nanofiber scaffolds due to ‎containing cell adhesion domains caused by‏ ‏their protein nature and because of their slow degradation, suitable ‎physical‏ ‏structure and mechanical properties are suitable candidates for articular‏ ‏cartilage repair.‎

Keywords

Journal of Textile Science and Technology
Volume 8, Issue 4 - Serial Number 32
December 2020
Pages 69-77

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History

  • Receive Date: 28 May 2019
  • Revise Date: 09 July 2019
  • Accept Date: 25 January 2020

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